Fluorescence In Vivo ImagingFluorescence in vivo imaging works on the basis of fluorochromes that are excited by an external light source, and which emit light of a different wavelength in response. The emitted fluorescence can be detected by using a camera which is sensitive in the spectral range of near-infrared. An example is the detection of cancer cells in the lymph nodes of rats. For this purpose, a fluorescence labelled dye is applied intravenously into rats. The detection of the weak emitted fluorescence, which permeates the tissue, requires a highly sensitive camera together with a specific filter.
On the left hand side, a light scatter image of a rat. The picture on the right hand side shows the overlay picture of light scatter image and fluorescence detection of the fluorescence-labelled dye accumulated in the tumour tissue in lymph nodes using the camera GE 1024 1024 DD NIR with a specific filter. The fluorescence around the head of the rat is due to the auto-fluorescence of the fur.